UG 100
Sequencing Platform
Unleash the power of omics at scale.
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UG 100 Sequencing Platform:
Two configurations.
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UG 100
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UG 100 Plus
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Compare the UG 100 and the UG 100 Plus
| UG 100 (ePCR) | UG 100 Plus (RAMP) | |
|---|---|---|
| System Specifications | ||
| Wafer capacity | 6 | 6 |
| Per-run wafer processing | Dual wafer | Dual wafer |
| Output per Sequencing Run | ||
| PF reads per wafer (billion) | 10 | 16 to 20 |
| PF reads per sequencing run (billion) | 20 | 32 to 40 |
| Output per sequencing run (150bp)a | 3.0 Tb | 5.4 Tb |
| Output per sequencing run (200bp)a | 4.0 Tb | 7.2 Tb |
| Output per sequencing run (300bp)a | 6.0 Tb | 10.8 Tb |
| Sequencing Run Time | ||
| ~150bp Read lengthb | ~7.0 hrs | ~7.0 hrs |
| ~200bp Read lengthb | ~9.0 hrs | ~9.0 hrs |
| ~300bp Read lengthb | ~12 hrs | ~12 hrs |
| Weekly Output | ||
| Wafers per weekc (150bp Read length) | 28 | 38 |
| Wafers per weekc (300bp Read length) | 24 | 24 |
| Reads per weeka (150bp Read length) | 280 B | 684 B |
| Reads per weeka (300bp Read length) | 240 B | 432 B |
| Output per weeka,c | ~72 Tb | ~130 Tb |
| Application Throughput Per Week | ||
| 30x WGSa,d | 675 genomes | 1,350 genomes |
| 10x Flex v1a,e | 27 M cells | 54 M cells |
a Based on 18B read per wafer throughput. Output depends on application, library quality, loading efficiency, and other experimental factors. A single flow cycle sequentially dispenses each distinct nucleotide (T, G, C, A) and correlates to 4 flows. Mostly natural sequencing-by-synthesis (SBS) flow sequencing does not result in only a single incorporated nucleotide in each sequencing colony in each flow and hence a simple average read length; instead, any number of nucleotides (e.g., 0, 1, 2, 3, 4, etc.) may be incorporated in each sequencing colony in each flow, resulting in a normal distribution of raw reads that is used to determine average read length.
b Runtime for sustained sequencing on the UG200™ Series sequencers; initial wafer loading adds ~2.3 h to sequencing runtime.
c Max productivity is achieved via a 5-day, single shift (8am - 5pm) work week with 7-day automated continuous sequencing enabled. Resultant wafer output is theoretical max productivity with 100% up time.
d 30x WGS = 400M reads/sample (~120Gb)
e 10x Flex v1 = 20k cells per sample * 10k reads per cell
UG 100 Technology
Open wafer substrate
Enables transformative low cost and scalability
The UG 100’s revolutionary sequencing architecture replaces complex and expensive flow cells with a 200mm open wafer, a staple from the semiconductor industry. This innovation unlocks multiple improvements to reduce cost, increase performance, and provide a roadmap for scalability.
24/7 sequencing operation
Built-in automation for continuous data generation
Featuring built-in automation and hot swap reagent and consumable capability. The UG 100 can run continuously to maximize data generation, minimize operator touch points and support flexible workflows on the 10 - 12 billion read wafers.
Rotational motion
Efficient, fast, and high-throughput
By using our open wafer and rotational motion to image, instead of micro-fluidics and cartesian scanning, the UG 100 Sequencer imparts stability and efficiency for both contamination-free reagent delivery and rapid imaging.
Unique flow-based chemistry
Ultima's unique single base flow chemistry was built for speed, efficiency, and accuracy where you need it most. Our chemistry inherently provides advantages for calling SNVs by asking how many nucleotides are added, but the base identity is never in question. Incorporating one type of nucleotide per flow enables an extremely low base substitution error rate.
Learn about Flow Based SBS Chemistry
ppmSeq for even more accurate SNV calling
ppmSeq, or paired plus minus sequencing, is Ultima's ground-breaking method to achieve accuracy of one part per million or better for calling single nucleotide variants (SNVs). ppmSeq combines the strong base calling performance of Ultima's flow chemistry with automated emulsion-based clonal amplification to sequence, identify and remove disagreement between both strands of a captured DNA molecule caused by sample error. Achieve extreme SNV quality without needing to over sequence.
Discover ppmSeq for rare event detection
SNVQ is the single nucleotide variant quality (SNVQ) score, which is different from a standard base quality score. The SNVQ score represents the error probability of the specific base substitution (e.g., A>G) rather than the aggregate error probability of any substitution (e.g., A>C/G/T).
Accurate homopolymer measurement
Ultima's UG 100 solves the homopolymer challenges that limited previous flow-based chemistries. By using steady state imaging and modern machine learning techniques, Ultima's chemistry performs well in measuring homopolymers so that you can now benefit from an extreme level of SNV accuracy with a high indel quality even with non-terminating chemistry.
Indel calling accuracy F1 per homopolymer indel length.
An ecosystem of compatible applications for the UG 100
Library prep
Our Solaris library prep workflows are designed with simplicity, flexibility and ease of use in mind, offering seamless compatibility with leading third-party library prep providers. Ultima supports an open and collaborative ecosystem offering you the freedom to choose tools and workflows that best fit your application needs for impactful research.
Explore Solaris workflowsAnalysis and bioinformatics
Leverage a suite of pre-configured pipelines optimized for Ultima data to efficiently execute analysis tasks in parallel with sequencing with no effect on sequencing throughput. The UG 100's dedicated high-performance compute Server performs read and sample-level secondary analysis and outputs file formats compatible with third-party software tools.
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Certified Service Providers
Access cost-effective and scalable sequencing through our growing network of global service providers vetted for technical expertise and adherence to best practices. Submit samples and start generating data on the UG 100 now.
Sequence with a CSP todayTechnology Access Program
Our Technology Access Program offers accessible entry to the UG 100 through our in-house applications lab. Sequence on our high-capacity fleet of UG 100 Sequencers and receive support at every step, from consultation through data analysis.
Discover our TAP offeringsBring UG 100 into your lab
Interested in exploring how your lab can benefit from high-throughput, cost-effective sequencing? Learn how our novel use of open wafers and built-in automation is continuously driving the cost of sequencing down to accelerate omics at scale.
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